Tendamistat
WebFeb 5, 1992 · The specific stability of tendamistat is similar to that of modified bovine pancreatic trypsin inhibitor, with only two of the native three disulfide bridges intact. This … WebThe Tendamistat secretion mediated by the different promoter constructions above varied dramatically in up to 10 mg/l in the case of the synthetic promoter and the aph promoter, and up to 500 mg/l mediated by the ermE-up promoter. The melC promoter allowed about 200 mg/l Tendamistat secretion and the tipA promoter proved to be inducible from ...
Tendamistat
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WebTEMPOL, the soluble spin-label 4-hydroxy-2,2,6,6-tetramethyl-piperidine-1-oxyl, has been used to determine the surface characteristics of tendamistat, a small protein with a well-characterised structure both in solution and in the crystal. A good correlation has been found between predicted regions of exposed protein surface and the intensity attenuations …
WebPeptide analogues of Tendamistat which include the most essential residues linked by novel ω-amino acids (X,Y,Z: H 2 N-(CH 2) n-CO 2 H, where n=2-10) were designed, synthesized (Ac-Tyr 15-X-Trp 18-Arg 19-Tyr 20-Y- Thr 55-Z-Asp 58-Gly 59-Tyr 60-Ile 61-Gly 62-NH 2), and analyzed for α-amylase inhibitory activity.Native dipeptide spacers … WebJan 1, 2006 · Tendamistat, a small disulfide-bonded β-sheet protein, and its three single/double-disulfide mutants are investigated by using a modified Gō-like model, aiming to understand the folding mechanism of disulfide-bonded protein as well as the effects of removal of disulfide bond on the folding process.Our simulations show that tendamistat …
WebIt has 29.6 % overall amino acid sequence identity to the well-known α-amylase inhibitor tendamistat. Here, we report the high-resolution NMR structure of parvulustat and compare it with that of tendamistat. The results suggest that parvulustat binds to α-amylase either by an induced-fit mechanism or a slightly different binding mode. WebIn our studies of structure-function correlation of polypeptides we used Tendamistat (TM), an α-amylase-inhibitor of Streptomyces tendae, as a model t High yield fermentation and …
WebThe active site of tendamistat coincides with the largest surface patch accessible to the probe. A strong hydration of protein N and C termini can also be suggested by this structural approach, as these locations exhibit reduced paramagnetic pertubations. Provided that the solution structure is known, the use of this paramagnetic probe seems to ...
WebMay 14, 1990 · Restrained Energy Refinement with Two Different Algorithms and Force Fields of the Structure of the Alpha-Amylase Inhibitor Tendamistat Determined by NMR in Solution Billeter, M., Schaumann, T., Braun, W., Wuthrich, K. (1990) Biopolymers 29: 695 drawers inside fireplaceWebTendamistat, produced by Streptomyces tendae, is a 74 amino acids α-amylase inhibitor which can target a wide range of mammalian α-amylase to inhibit the hydrolysis of starch. It is a small size molecule with considerable stability, and the structural data is also available via high resolution NMR and X-ray analysis. employeeservive.smf.com:8000/nwbcWebInhibition of the enzyme's activity would reduce glucose absorption by the small intestine. Our objective was to develop small peptides based on essential binding elements of the natural protein inhibitor, Tendamistat. These smaller analogs may be better studied structurally and conformationally to help us understand molecular-level interactions. drawers intimates \u0026 toysWeb这组词都有“混合”的意思，其区别是： mingle: 暗示混合后的各成分仍保持各自的特性，能辨别出来。 merge: 指一种成分被别一种成分吸收或融合，着重成分的个性消失在整体之中。 blend: 一般可与mix和mingle换用。混合与其各成分的性质通常是一致的，侧重混合整体的统一性与和谐性。 employee service university of iowaWebJul 16, 2003 · The crystal structure of the proteinaceous alpha-amylase inhibitor tendamistat has been determined at 100 K to a resolution of 0.93 A. The final R factor … drawers intimatesWebTendinitis is an inflammation of tendons. Tendons join muscles to bones. Tendinitis can occur in the fingers, thumbs, wrists, elbows, knees, and thighs. The most likely cause is … drawers into wall shelvesWebEngels’ Forschungsinteresse galt überwiegend der Nucleotidchemie; insbesondere der Synthese modifizierter Oligonucleotide und deren Analytik und möglichen therapeutischen Anwendungen sowie der Funktion von Parvulustat und Tendamistat. Joachim Engels hat über 300 wissenschaftliche Publikationen und Patente verfasst. drawers in your freezer