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Pylsgrna-osu6a

TīmeklisPlant multiplex genome editing vector pYLsgRNA-OsU6a/LacZ Taxonomy ID: 1648247 (for references in articles please use NCBI:txid1648247) current name. Plant … http://m.hedgehogbio.net/Products/D8661079.html

多靶点pCRISPR载体(单子叶和双子叶植物用)使用方法精编版

Tīmeklis2024. gada 21. febr. · pEBB-3XMyc-TRAF2(Plasmid #44104) 慢病毒质粒 真核表达质粒 原核表达质粒 报告基因质粒 自噬质粒 双分子荧光 抗体表达质粒 基因辑质粒， 酵母相关质粒 植物相关质粒， 昆虫相关质粒 shRNA质粒 枯草芽孢杆菌质粒 现货质粒，货期短，发货及时_pEBB-3XMyc-TRAF2(Plasmid #44104)_质粒宝_上海海吉浩格生物科技 … Tīmeklis2016. gada 19. febr. · Vectors (pYLsgRNA-OsU3, pYLsgRNA-OsU6a, pYLsgRNA-OsU6b, and pYLsgRNA-OsU6c for rice and pYLsgRNA-AtU3b, pYLsgRNA-AtU3d, … find me a 2022 honda crv https://highland-holiday-cottage.com

miRNA408在调控作物镉积累中的应用 - X技术

http://www.labbase.net/Product/ProductItems-27-167-2187-80105.html Tīmeklis2015. gada 14. apr. · 为了提高构建好的多靶点载体的稳定性，避免在农杆菌或者植物基因组中sgRNA表达盒之间发生同源重组，从水稻克隆了4个不同snRNA启动 … Tīmeklis2016. gada 19. febr. · Vectors (pYLsgRNA-OsU3, pYLsgRNA-OsU6a, pYLsgRNA-OsU6b, and pYLsgRNA-OsU6c for rice and pYLsgRNA-AtU3b, pYLsgRNA-AtU3d, … er diagram using crow\u0027s foot notation

A Robust CRISPR/Cas9 System for Convenient, High

Category:Genetic Manipulation of Reactive Oxygen Species (ROS ... - Springer

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Pylsgrna-osu6a

miRNA408在调控作物镉积累中的应用 - X技术

Tīmeklis2024. gada 2. jūl. · The arrangement of sgRNA cassettes driven by U6 promoter-was as follows; LacZ-OsU6a-T1-OsU6b-T2 with corresponding sizes of 629 bp and 515 bp for T 1 and T 2 respectively (Supplementary file 1, ... pYLsgRNA-OsU6a (3289 bp); (b) cloning strategy of PYLsgRNA-OsU6a showing sticky ends created by cutting with … Tīmeklis2024. gada 23. janv. · Briefly, two short guide RNAs (sgRNAs) targeting OsPLGG1a and OsPLGG1b were cloned into pYLsgRNA-OsU6a and pYLsgRNA-OsU6b, …

Pylsgrna-osu6a

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http://www.app17.com/c145959/products/d11302689.html TīmeklisPlasmid pYLsgRNA-OsU6a from Dr. Yao-Guang Liu's lab is published in Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007. This …

Tīmeklis2024. gada 24. jūl. · pYLsgRNA-OsU6a/LacZ. catalog : 66195. more info or order : Addgene product webpage. citations: 2. Reference; Ma X, Liu Y. CRISPR/Cas9 … http://www.shhebio.com/page53?article_id=59816

Tīmeklis2024. gada 8. dec. · 取10μm靶位点前后引物各5μl混合后99℃退火5min并自然冷却获得含敲除靶位点的双链序列，然后利用bas1(neb公司)边酶切边连接(37℃)，将靶位点序列连至中间载体pylsgrna-osu6a上，随后利用通用扩增引物ctccgttttacctgtggaatcg(uf)和cggaggaaaattccatccac(gr-r)分别与靶位点后引物和前 ... Tīmeklis本发明公开了通过抑制SD1基因的表达降低四倍体野生稻株高的方法。本发明的具体实施例证明通过编辑绿色革命基因SD1，可实现株高的快速驯化，能使四倍体野生 …

Tīmeklis2024. gada 11. jūn. · Briefly, the 20-nt target sequences were synthesized using overlapping PCR. In the first round of PCR, the 20-nt target sequences were ligated …

Tīmeklis2015. gada 3. aug. · By Omega-PCR cloning, an E. coli promoter–LacZ sequence (198 bp in length) was inserted into pYLsgRNA-OsU6a and pYLsgRNA-AtU3b, to … er diagram topicshttp://www.bioon.com.cn/reagent/Show_product.asp?id=4116063 erdine chisholm obituaryTīmeklis2015. gada 14. sept. · 194. Plant multiplex genome editing vector pYLsgRNA-OsU6a, complete sequence 3,289 bp circular other-genetic KR029105.1 GI:816206591. 195. … find me a 5000 unsecured bad credit loanTīmeklis2024. gada 4. jūn. · pYLsgRNA intermediates (PYLsgRNA-OsU6a/Lac, PYLsgRNA-OsU6b, PYLsgRNA-OsU6c, and PYLsgRNA-OsU3). 2. pYLCRISPR/Cas9P ubi-H binary vector. 3. Restriction enzymes. 4. T 4 DNA ligase. 5. Gibson Assembly Cloning Kit. 6. 2 × Taq Master Mix. 7. Chimeric primers with target sequence and universal … er dictionary\u0027sTīmeklis2024. gada 10. jūn. · (c) PCR GXL of gRNA expression cassettes; pYLsgRNA-OsU6a and pYLsgRNA-OsU6b ampli ed using primers gR-R/gRNA target adapter forward primer for reaction 1, U-F/gRNA target adapter RP in reaction 2. er diagram weak entity exampleTīmeklis2024. gada 21. febr. · pMSCV-CasRx-gRNA-puro 慢病毒质粒 真核表达质粒 原核表达质粒 报告基因质粒 自噬质粒 双分子荧光 抗体表达质粒 基因辑质粒， 酵母相关质粒 植 … find me a 24 hour pharmacyTīmeklis2024. gada 7. janv. · Ma X, Liu Y. CRISPR/Cas9-Based Multiplex Genome Editing in Monocot and Dicot Plants. Curr Protoc Mol Biol. 2016;115:31.6.1-31.6.21 pubmed … e.r dickson field trip at fox 10 news