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Pchf3-eyfp

SpletThe pCambia2300 vector offers: High copy number in E.coli for high DNA yields. pVS1 replicon for high stability in Agrobacterium. Small size. Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest. Bacterial selection with kanamycin. Plant selection with kanamycin. Splet05. apr. 2024 · The mechanisms underlying ETS-driven prostate cancer initiation and progression remain poorly understood due to a lack of model systems that recapitulate this phenotype.We generated a genetically engineered mouse with prostate-specific expression of the ETS factor, ETV4, at lower and higher protein dosage through mutation of its …

S0199-9 AAV2/9-hEF1a-DIO-hChR2(H134R)-EYFP-WPRE-pA - 上海 …

Splet26. mar. 2024 · Genetic strategies using Cre-dependent mouse strains, especially the Ai32 line expressing Channelrhodopsin (ChR2)-EYFP fusion protein, have been a popular means to drive opsin expression in a cell-type specific manner. Here we report a low level of leaky 'off-target' (Cre-independent) ChR2-EYFP expression in Ai32/Ai32 homozygous mice … Splet007612 - Thy1-ChR2-YFP Strain Details. Technical Information Scientists. Overview. How it's Used. my maps hullcc https://highland-holiday-cottage.com

(PDF) Tartary Buckwheat R2R3-MYB Gene FtMYB3 Negatively

Splet26. dec. 2024 · EYFP颜色很亮,现在仍在使用。但其抗酸性差、对氯化物敏感。对其改造的突变体有mCitrine和mVenus。 3.红色荧光蛋白 主要基于对红色荧光蛋白DsRed的改造。使用较广泛的有mCherry,mStrawberry,dTomato等。 4.蓝色荧光蛋白 SpletV68L is included in native EYFP, and we therefore opted to not include F64L in our new Cl2 sensor. Since V163A may also be involved in eliminating chloride sensitivity in Venus by presumed shortening of the side chain, we introduced V163S, with a longer side chain that enhances halide sensitivity [10] while simulta-neously maintaining ... Splet质粒类型: 植物载体. 联系我们. 全国服务热线:0532-83868918 业务电话: 15201165768 商务邮箱: [email protected] 商务qq: 3555477400 联系地址:山东省青岛市黄岛区珠海街道灵山湾路3213号龙泰综合楼316-1 my maps bedfordshire

Genome-wide identification of MAPK gene family members in

Category:EYFP :: Fluorescent Protein Database

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Pchf3-eyfp

Addgene: pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Splet20. nov. 2024 · Although ubiquitous in biological studies, the enhanced green and yellow fluorescent proteins (EGFP and EYFP) were not specifically optimized for neuroscience, … Splet03. dec. 2010 · 检测GFP、EGFP、YFP、EYFP、CFP的GFP抗体. GFP是绿色萤光蛋白(Green Fluorescent Protein)的简称,由238个氨基酸残基组成。GFP蛋白质最早是由下村脩等人在1962年在一种学名Aequorea victoria的水母中发现。其基因所产生的蛋白质,在蓝色波长范围的光线激发下,会发出绿色萤光。

Pchf3-eyfp

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Splet26. mar. 2024 · Optogenetics enables the selective activation of genetically-targeted neuronal populations using light-sensitive ion channels. Genetic strategies using Cre-dependent mouse strains, especially the Ai32 line expressing Channelrhodopsin (ChR2)-EYFP fusion protein, have been a popular means to drive opsin expression in a cell-type … http://www.hedgehogbio.net/Products/D11840028.html

SpletEYFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer with high acid sensitivity. compare. … SpletIn addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2 (H134R)-EYFP-WPRE-HGHpA plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

http://www.biofeng.com/zaiti/zhiwu/pcambia35s-EYFP.html SpletpCHF3-UBQ10-YFP黄色荧光UBQ10启动子-质粒载体-ATCC-DSM-CCUG-泰斯拓生物. 产品名称. pCHF3-UBQ10-YFP黄色荧光UBQ10启动子. 商品货号. TSPLA10837. 基本信息. 使用说明. 质粒干粉 (常温运输,存于-20度,90天保质期,请务必先转化提质粒后使用) ①收到质粒干粉后请先5000rpm离心1min ...

Splet13. maj 2013 · 它用的是EYFP的1-154和155-238aa片段分别连在欲检测蛋白一端(还进行了一系列的试验寻找最佳可行的分割片段),如果蛋白相互作用,两个YFP片段靠近,激发产生荧光。. 其后的几篇文章大多均采用此文章的方法。. 只有在04年PNAS的一篇文章,介绍了 …

Splet27. maj 2024 · Taken together, our results indicate that fusing eYFP C-terminally to MinE impairs its ability to displace MinC from MinD, to directly bind to the membrane and to stimulate MinD ATPase activity, and renders the protein prone to aggregation. The results are likely to be generalizable to other fluorescent proteins beyond eYFP. 2. Results 2.1. my maps lethamyrhttp://www.hedgehogbio.net/Products/D11302699.html my maps google comSplet15. maj 2024 · NRAMPs were involved in one or several metal(s) transport, including Fe, Mn, Zn, Al and/or Cd (Peris-Peris et al., 2024).In this study, expression of TtNRAMP6 in the Cd … my maps haringeySplet载体基本信息. 出品公司: 爱迪基因. 载体名称: pCas9-GFP 或 pCas9_GFP. 质粒类型: CRISPR/Cas9载体;基因敲除载体;哺乳动物细胞载体;双顺反子载体. 高拷贝/低拷贝: … my maps hull city councilSpletThe enhanced yellow fluorescent protein gene used in these studies contains four amino acid substitutions that shift the emission maximum of green fluorescent protein ( GFP) by 18 nanometers, from approximately 509 to 527 nanometers. The gene is optimized with human codons and features a consensus Kozak translation initiation signal to achieve ... my maps instructionsSplet产品名称. pCHF3-UBQ10-YFP黄色荧光UBQ10启动子. 商品货号. TSPLA10837. 基本信息. 使用说明. 质粒干粉 (常温运输,存于-20度,90天保质期,请务必先转化提质粒后使用) ① … my maps in googlehttp://www.biofeng.com/zaiti/CRISPR-Cas9/pCas9-GFP.html my maps walsall