2024 Line1 orf2 antibody

Line1 orf2 antibody

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Nettet10. des. 2014 · L1 mobilization relies on the function of two, self-encoded proteins, ORF1 and ORF2. The ORF2 protein contains two characterized domains: endonuclease and … NettetORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, …

Endonuclease Sigma-Aldrich

NettetORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, … NettetGeneral description. LINE-1 retrotransposable element ORF2 protein (UniProt: O00370; also known as EC:2.7.7.49, ORF2p endonuclease, ORF2p) is encoded by the Orf2p … snow custom flooring

LINE-1 vectors mediate recombinant antibody gene transfer by ...

NettetHome - Springer NettetAn active LINE-1 encodes for 2 proteins translated from a single RNA containing two non-overlapping ORFs, ORF1 and ORF2. ORF2p is described in this entry as a … NettetAnti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) at 1/1000 dilution + mouse testis lysate at 20 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution … snow customs

LINE-1 ORF2p抗体 Abcam中文官网

NettetORF1p and ORF2p are LINE1 encoded proteins essential for LINE1 retrotransposition. LINE1s are silenced epigenetically in somatic tissues, and their reactivation has been associated with cancer pathogenesis. Here, we present evidence that nucleolin (NCL) regulates expression of LINE1-ORF1p (L1-ORF1p) in NSCLC cells. Nettet1. aug. 1998 · Heterologous expression of human LINE-1 ORF2 in yeast yielded a single polypeptide (M r 145 000) which reacted with specific antibodies and co-purified with a reverse transcriptase activity not present in the host cells. Various deletion derivatives of the ORF2 polypeptide were also synthesized. snow cut rv parkNettetAntibodies that detect L1/ORF2 can be used in several scientific applications, including ELISA and Western Blot. These antibodies target L1/ORF2 in Mouse, Human and … snow cut park wilmington nc

"NettetAnti-LINE1 ORF2 Antibody, clone 1G4E11. US ES. Aplicaciones Productos Servicios Asistencia técnica. Small Pack Size Antibodies; MABE1987; All Photos (1) … " - Line1 orf2 antibody

Line1 orf2 antibody

Nettetwww.ncbi.nlm.nih.gov NettetImmunogen. Synthetic peptide corresponding to Human LINE-1 ORF2p conjugated to keyhole limpet haemocyanin. Two synthetic peptides conjugated to keyhole limpet …

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NettetORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons. Limitations NettetAnti-LINE-1 Retrotransposable Element ORF2 Protein [1G4E11] Antibody. This mouse IgG1k monoclonal antibody was generated against bacterially-purified human …

NettetAnti-Orf2 is directed against mouse ORF2 near a c-terminus position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide … NettetLINE-1 vectors mediate recombinant antibody gene transfer by retrotransposition in Chinese hamster ovary cells Biotechnol J. 2024 May 3;e2000620. doi: 10.1002 ... (ORF1 and ORF2) of LINE-1. Genomic PCR analysis detected the transgene sequence in almost all tested clones.

NettetLINE-1 (L1) is a class of autonomous mobile genetic elements that form somatic mosaicisms in various tissues of the organism. The activity of L1 retrotransposons is strictly controlled by many factors in somatic and germ cells at all stages of ontogenesis. NettetAnti-LINE1 ORF2 Antibody, clone 1G4E11 Anti-LINE1 ORF2, clone 1G4E11, Cat. No. MABE1987, is a mouse monoclonal antibody that detects LINE-1 retrotransposable element ORF2 protein and is tested for use in ELISA, Inhibition Assay, and Western Blotting. - Find MSDS or SDS, a COA, data sheets and more information.

Nettet31. des. 2024 · To test whether ORF2p could be detected with specific reagents, we developed and validated five rabbit monoclonal antibodies with immunoreactivity for …

Nettet25. feb. 2024 · Full-length LINE1 encodes two proteins essential for LINE1 retrotransposition: L1ORF1p and L1ORF2p. L1ORF1p is a nucleic acid chaperone with high RNA-binding affinity [ 13 ]. L1ORF2p harbors endonuclease (EN) and reverse-transcriptase activities and a cysteine-rich domain that is also essential for … snow cute girlsNettet26. jan. 2016 · LINE-1 (L1) retrotransposons are a source of endogenous reverse transcriptase (RT) activity, which is expressed as part of the L1-encoded ORF2 protein … snow cute outfitsNettet28. aug. 2024 · The kinetics of nascent LINE-1 transcription was consistent with changes at the protein level, as determined by immunofluorescence with an antibody to ORF1p ( Fig. 1d ). ORF1p exhibited the... snow cutting practiceNettetAnti-LINE1 ORF2 Antibody, clone 1G4E11 Match Criteria: Product Name, Keyword All Photos ( 2) Monoclonal Anti-KLF2 antibody produced in mouse Match Criteria: Product … snow cutter in japanNettet9. jun. 2013 · The L1 expresses a 6-kb bicistronic RNA that encodes the 40 kDa Open Reading Frame-1 RNA-binding protein (ORF1p) of essential but uncertain function, and a 150 kDa ORF2 protein with endonuclease and reverse transcriptase (RT) activities ( … snow cutting cordNettetAnti-LINE-1 ORF2p antibody 製品の詳細 Chicken polyclonal to LINE-1 ORF2p 由来種 Chicken 特異性 The two synthetic immunogen peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein. This antibody is intended for use in studying control of L1 retrotransposons. アプリケーション適用あり: ELISA, WB more … snow d\u0027occasionNettetImmunocytochemistry/ Immunofluorescence: LINE-1 ORF1p Antibody [NBP2-66934] - Staining L1ORF1p in F9 cells (green). Cells were fixed in paraformaldehyde, … snow cyber security