WebFeb 10, 2024 · SF188, KNS42, GIN28 and HeLa cells were plated on 8-well chamber slides at optimised densities and incubation periods. Cells were washed in PBS, fixed in 0.4% paraformaldehyde for 20 min, permeabilised with PBS-T (0.1% tween, 0.25% triton-X in PBS) and blocked with 5% NGS in PBS-T for 1 h at room temperature. WebThe KNS42 cell line was maintained in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin and incubated at 37 °C in 5% CO 2. The cells were treated with 10 μM of compound 15. The cells were washed with 1× PBS, scraped with a cell scraper and centrifuged to collect cell ...
Profiling cytotoxic microRNAs in pediatric and adult glioblastoma cells …
WebNov 5, 2024 · Interestingly, we evidenced that the mutation confers an increased resistance to ionizing radiations in Res259 and KNS42 cells. Moreover, we showed that H3.3K27M … WebCell line name: KNS-42: Synonyms: KNS42: Accession: CVCL_0378: Resource Identification Initiative: To cite this cell line use: KNS-42 (RRID:CVCL_0378) Comments: Part of: Cancer … long lawford primary
Cancers Free Full-Text Lipoprotein Deprivation Reveals a ...
WebFeb 18, 2024 · KNS42 cells were more sensitive with significant growth inhibition at all times tested with 10 and 20 µg/mL of DHMEQ, reaching 70% inhibition after 72 h. SF188 cells showed a moderate reduction of proliferation after 24 h in relation to the control (25% at the maximum concentration); however, after 48 h, all treatments above 2.5 µg/mL showed ... WebWe also used KNS42 cells, an H3.3(G34V) pediatric high-grade glioma cell line, to show that Rpp29 1) represses H3.3 incorporation into transcriptionally active protein-coding, rRNA, and tRNA genes; 2) represses mRNA, protein expression, and antisense RNA; and 3) represses euchromatic post-translational modifications (PTMs) and promotes ... WebAug 18, 2024 · To determine how lactate drives survival of GBM cell cultures, we took a two-pronged strategy by performing a broad polar metabolite in KNS42 cells and transcriptome analysis in GBM22, GBM43, and KNS42 cells, utilizing nutrient-deprived conditions in the presence or absence of lactate (Figures 2 A and 2B; Table S1; Figures S2 … hop am memory