Jc-1 red/green ratio
WebMay 22, 2006 · The aggregated form of JC-1 molecules (red fluorescence) accumulated in functional mitochondria. On the other hand, the green fluorescence signal from JC-1 monomers is evenly distributed in the cytoplasm (Fig. 1 A and B).In the control groups, the red/green ratio was 18.2 ± 9.3, while in the hypoxia/re-oxygenization group this ratio was … WebJC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications. A cationic, fluorescent, carbocyanine dye …
Jc-1 red/green ratio
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WebMitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨ m induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a ... WebAt higher potentials, JC-1 forms red-fluorescent (λ em 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density. Packaging 5 mg Safety Information Storage Class Code
WebFeb 8, 2024 · The mitochondrial membrane potential (MMP) was measured by flow cytometry using MitoProbe JC-1 (A and B). The histogram shows the ratio of JC-1 … WebApr 1, 2024 · In contrast, the LRAR or LRsBAR-treatment completely restored the MMP by increasing the JC-1 red/green ratio to 0.98–0.99. At the same time, the treatment of free Rutin and LR only elevated the JC-1 red/green ratio to 0.72 and 0.79, respectively. Similarly, LRAR or LRsBAR treatment also effectively scavenged ROS in N2a cells pre-treated with ...
WebJC-1 and JC-9 are cationic dyes (Figure 1) that exhibit potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~525 nm) to red … Webgreen (~529 nm) to red (~590 nm). Consequently, mitochon-drial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates.1-3 JC-1 can be used as an indicator of mitochon-
WebVarious types of ratio measurements are possible by combining signals from the green-fluorescent JC-1 monomer (absorption/emission maxima ~514/529 nm in water) and the red-fluorescent J-aggregates (emission maximum 590 nm), which can be effectively …
WebVarious types of ratio measurements are possible by combining signals from the green-fluorescent JC-1 monomer (absorption/emission maxima ~514/529 nm in water) and the J-aggregate (emission maximum 590 nm), which can be effectively excited anywhere between 485 nm and its absorption maximum at 585 nm. spotlight 28WebJC‐1 does not form aggregates in mitochondria’s with low ∆ψ M and remain in monomeric form and exhibit green fluorescence. Thus higher the ratio of red to green fluorescence, … spotlight 29 buffetWebCayman’s JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit can be used to study mitochondrial behavior in a variety of conditions, including apoptosis. Changes in Δψ M, reflected by aggregation level of JC-1, can be determined as a ratio of red to green mean fluorescence intensities using flow cytometry. spotlight 29 casino careersWebTo monitor mitochondrial membrane potentials, JC-1 dye was used. The ratio (red/green) of JC-1 was considered as an indicator of intact mitochondrial membrane potential. Flow cytometry was done to analyze extracellular mitochondria particles and their possible cellular origins. Results: shenango valley area scale modelersWebJan 26, 2004 · The fluorescent signal intensity at 590 nm (red) was divided by the intensity at 535 nm (green), and this red/green ratio was used to estimate the mitochondrial membrane potential (ΔΨ m ). Larger JC-1 red/green … spotlight 29 casino hostsWebApr 9, 2024 · The green (EX 485 nm and EM 535 nm) and red (EX 550 nm and EM 600 nm) emissions were used for measuring the ratio of red to green fluorescent. For the JC-1 experiments, there were four TETRA groups: vehicle-control (Meth), TETRA (30 µg/mL, 60 µg/mL and 120 µg/mL); and four CPFX groups: Vehicle-control (HCl), CPFX (30 µg/mL, 60 … spotlight 29 casino diningWebMar 30, 2016 · Cells were harvested and stained with MitoTracker Deep Red (MTDR) (A), JC-1 (B), or MitoTracker Green (MTG) (C), followed by flow cytometric analysis. The ratio of the red to green fluorescence intensity in JC-1 stained cells was used to represent mitochondrial membrane potential. spotlight 29 casino hotels rooms