Hoechst structure
Nettet18. nov. 2024 · Hoechst is a substrate of multidrug transporters, which translocate a wide range of structurally unrelated compounds from cells in a metabolic energy-dependent … Nettet1. feb. 1999 · Although the structures of Hoechst 33258 and netropsin are ostensibly quite dissimilar, examination of the molecular recognition surface components of the two ligands directed towards the DNA minor-groove receptor reveals significant similarities.
Hoechst structure
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NettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP strategy, is detected at two emission wavelengths after UV excitation: Hoechst Red (630–650 nM) and Hoechst Blue (405–450 nM). NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish …
NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … NettetHoechst 33258 C25H27Cl3N6O CID 31953 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, …
NettetHoechst and DAPI are popular blue fluorescent, nuclear-specific dyes that can be used to stain live or fixed cells. Both DAPI and Hoechst are minor-groove binding dyes with a … NettetDefinition of Hoechst in the Definitions.net dictionary. Meaning of Hoechst. What does Hoechst mean? Information and translations of Hoechst in the most comprehensive …
NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish …
Nettet3. mar. 1988 · The binding of Hoechst to DNA is stabilized by a combination of hydrogen bonding, van der Waals interaction and electrostatic interactions. The binding preference for AT base pairs by the drug is the result of the close contact between the Hoechst molecule and the C2 hydrogen atoms of adenine. brian chrystalNettet1. jan. 2009 · Loonteins et al. [3] suggested four interactions between DNA and Hoechst 33258, could be mediated by DNA sequence, charge on dye, dye interactions and structure of the dye. Sequence-mediated binding provides specificity in that Hoechst dyes bind in the minor groove requiring a specific sequence (often four A-T base pairs). coupon for jamplayHoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd compound made by the company. There are three related Hoechst stains: Hoechst … Se mer Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue-cyan fluorescent light around an emission spectrum maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the … Se mer Because Hoechst stains bind to DNA, they interfere with DNA replication during cell division. Consequently, they are potentially mutagenic and carcinogenic, so care should be used in … Se mer • Spectral traces for fluorescent dyes • Manual for Hoechst stains • An online guide to fluorescent probes and commercial labeling technologies Se mer A concentration of 0.1–12 μg/ml is commonly used to stain DNA in bacteria or eukaryote cells. Cells are stained for 1-30 min at room temperature or 37 °C and then washed to … Se mer • Bisbenzimide • Carcinogen • DAPI • DNA-binding protein Se mer coupon for jared jewelersNettetRamipril was synthesized by chemists at the German chemical firm Hoechst. It was patented in 1981 and approved as a prescription drug eight years later. The white … brian christy realtorNettetPreparing Hoechst dye stock solution 1. Prepare the Hoechst dye stock solution by dissolving the contents of one vial (100 mg) in 10 mL of deionized water (diH 2 O) to create a 10 mg/mL (16.23 mM) solution. Note: Hoechst dye has poor solubility in water, so sonicate as necessary to dissolve. brian chronikNettetChemical structure of Hoechst stains. Like DAPI, Hoechst dyes preferentially bind to the adenine-thymine-rich sequences in the minor grooves of double-stranded DNA. Upon binding, their fluorescence … coupon for jansen towable backhoeNettetCell Structure Reagents and Kits Hoechst 34580 Invitrogen™ Hoechst 34580 Catalog number: H21486 Related applications: Cell Structure Cell Tracing & Tracking Cell Viability, Proliferation & Function Cellular Imaging Technical Support Customer Service Add to cart Product Overview Figures Recommendations Documents FAQ Citations & … coupon for jbhifi